Jove
Visualize
Contact Us

Related Experiment Videos

Linker histone H1M transcripts mark the developing germ line in zebrafish.

Karin Wibrand1, Lisbeth Charlotte Olsen

  • 1Sars International Centre for Marine Molecular Biology, Bergen High Technology Centre, Thormøhlensgt 55, N-5008 Bergen, Norway.

Mechanisms of Development
|September 3, 2002
PubMed
Summary

Zebrafish germ cell development relies on maternal factors. New research identifies linker histone H1M transcripts as early markers for the germ line from gastrulation to 18 hours post-fertilization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MicroRNA-34a Acutely Regulates Synaptic Efficacy in the Adult Dentate Gyrus In Vivo.

Molecular neurobiology·2019
Same author

Evidence for a centrosome-attracting body like structure in germ-soma segregation during early development, in the urochordate Oikopleura dioica.

BMC developmental biology·2018
Same author

Widespread promoter methylation of synaptic plasticity genes in long-term potentiation in the adult brain in vivo.

BMC genomics·2017
Same author

Corrigendum: Dynamic expression of long noncoding RNAs and repeat elements in synaptic plasticity.

Frontiers in neuroscience·2016
Same author

Common variants in the ARC gene are not associated with cognitive abilities.

Brain and behavior·2015
Same author

Dynamic expression of long noncoding RNAs and repeat elements in synaptic plasticity.

Frontiers in neuroscience·2015
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Developmental biology
  • Genetics
  • Molecular biology

Background:

  • Maternally supplied factors are crucial for germ cell lineage establishment in lower vertebrates.
  • In zebrafish, germ cell-soma segregation is largely complete by the late blastula stage.

Purpose of the Study:

  • To identify novel factors involved in germ cell development in zebrafish.
  • To characterize early molecular markers of the zebrafish germ line.

Main Methods:

  • Subtractive cDNA cloning was employed to identify differentially expressed genes.
  • Analysis of gene expression patterns during early zebrafish development.

Main Results:

  • Linker histone H1M transcripts were identified as a novel marker.

Related Experiment Videos

  • These transcripts are specifically detected in the germ line from early gastrulation through 18 hours post-fertilization.

    • Conclusions:

    • Linker histone H1M serves as an early molecular indicator of the germ line in zebrafish.
    • This finding contributes to understanding germ cell specification mechanisms in vertebrates.