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Published on: September 1, 2017
[Determination of sarsasapogenin in Anemarrhena asphodeloides Bunge by GC]
1Department of Education Guarantee, Second Military Medical University, Shanghai 200433.
Abstract
Objective:
To determine sarsasapogenin in Anemarrhena asphodeloides.
Method:
Chloromethane extract (1 microliter) with cholesterol as internal standard was analyzed on HP-1 column, operated at 270 degrees C with N2 as carrier gas and FID.
Result:
The method was linear within the range of 0.245-2.94 micrograms.microliter-1, with a correlation coefficient of 0.9996. The average recovery was 95.52% +/- 1.77%,
Conclusion:
The method is reproducible, rapid and sensitive.
