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Updated: Sep 29, 2026
GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Published on: May 19, 2012
[Determination of oleanolic acid and ursolic acid in spica Prunellae by derivative GC method]
Objective:
To develop a GC method to determine the oleanolic acid and ursolic acid in Spica Prunellae.
Method:
Before GC analysis, the sample was derivatized with CH2N2 solution. The GC conditions were as follows: comumn-10% SE-30(2m x 3mm) and column temperature -270 degrees C.
Result:
The two constituents were well separated and had good linearity in the range of 0.0025-0.4000 mg/ml. The average recoveries and RSD of oleanolic acid were 93.53% and 3.5%, 94.18% and 3.0% respectively.
Conclusion:
The method is good for determining oleanolic acid and ursolic acid in Chinese medicines.
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