[Effect of human serum on the growth of Helicobacter pylori]
1Department of Infectious Diseases, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Abstract:
To investigate whether sheep blood could be replaced with human serum or fetal calf serum for Helicobacter pylori(Hp) culture, Hp was inoculated onto the culture plates containing 7% of sheep blood, human serum, or fetal calf serum respectively. The colonies were counted after 5 days of culture. Similar culture results were obtained in the plates containing sheep blood, human serum, or fetal calf serum respectively. No effect of anti-Hp-IgG antibody in human serum on the growth of Hp was found (P > 0.05). However, heat-inactivated human serum significantly suppressed the growth of Hp (P < 0.01). Human serum or fetal calf serum may be used for Hp culture where sheep blood is not available. Bactericidal activity against Hp in human serum is mediated by complements.
More Related Videos
10:44One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
Published on: June 18, 2016
05:23Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance
Published on: March 7, 2025
Related Concept Videos
Pathophysiology of Peptic Ulcer Disease: Injurious Factors
In the antrum region, G cells secrete the gastrin hormone that binds to gastrin-cholecystokinin-B (CCK2) receptors on parietal and enterochromaffin-like (ECL) cells in the fundic glands. Simultaneously, the vagus nerve releases acetylcholine, which binds to M3...
Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy
Microbiota of the Stomach and Small Intestine
Peptic Ulcer
Gastritis II: Pathophysiology
Peptic Ulcer Disease II: Pathophysiology
