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The ferredoxin docking site of photosystem I
Pierre Sétif1, Nicolas Fischer, Bernard Lagoutte
1CEA Saclay, Département de Biologie Joliot-Curie, Service de Bioénergétique and URA CNRS 2096, 91191 Gif sur Yvette, Cedex, France. setif@dsvidf.cea.fr
Abstract:
The reaction center of photosystem I (PSI) reduces soluble ferredoxin on the stromal side of the photosynthetic membranes of cyanobacteria and chloroplasts. The X-ray structure of PSI from the cyanobacterium Synechococcus elongatus has been recently established at a 2.5 A resolution [Nature 411 (2001) 909]. The kinetics of ferredoxin photoreduction has been studied in recent years in many mutants of the stromal subunits PsaC, PsaD and PsaE of PSI. We discuss the ferredoxin docking site of PSI using the X-ray structure and the effects brought by the PSI mutations to the ferredoxin affinity.