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The rat shorn mutation (shn) maps between D7Got143 and D7Rat94
R R Chrissluis1, T A Stoklasek, J A Loman
1Biological Sciences, Central Connecticut State University, New Britain 06050, USA.
Molecular Genetics and Metabolism
|September 5, 2002
Summary
The shorn (shn) rat mutation causes near-complete hair loss. Genetic mapping refined its location on Chromosome 7, suggesting a possible chromosomal rearrangement affecting recombination.
Area of Science:
- Genetics
- Animal Models
- Molecular Biology
Background:
- The recessive shorn (shn) mutation in rats results in hypotrichosis, an almost complete absence of hair.
- Previous studies localized the shn mutation to distal rat Chromosome 7, between the telomere and D7Mgh1.
Purpose of the Study:
- To further refine the genetic location of the shorn (shn) mutation on rat Chromosome 7.
- To establish a high-resolution meiotic map of microsatellite markers in the distal region of Chromosome 7.
Main Methods:
- Microsatellite polymorphism analysis was performed on a 184-member backcross rat panel.
- Meiotic ordering of 18 microsatellite markers was achieved across a 16.8cM region.
Main Results:
- The shn mutation was precisely mapped to a 2.7cM interval between markers D7Got143 and D7Rat94.
- Ten D7Got markers, previously mapped by radiation hybrid analysis, were meiotically ordered.
- The shn mutation could not be meiotically separated from 8 microsatellite markers, spanning a significant genetic distance.
Conclusions:
- The refined mapping of the shn mutation provides a valuable resource for further positional cloning efforts.
- The lack of recombination suggests the shn mutation may be associated with a chromosomal rearrangement that suppresses recombination in this region.