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Updated: Sep 29, 2026
Caspase-3 Activity in the Rat Amygdala Measured by Spectrofluorometry After Myocardial Infarction
Published on: January 12, 2016
Huperzine A attenuates apoptosis and mitochondria-dependent caspase-3 in rat cortical neurons
1State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 294 Tai-yuan Road, Shanghai 200031, PR China.
Abstract:
The neuroprotection of huperzine A against apoptosis was investigated. In cultures of rat primary cortical neurons, neuronal apoptosis was induced by serum deprivation for 24 h, which was accompanied by enhanced caspase-3 activity and the release of cytochrome c into the cytosol from mitochondria. Pretreating the neurons for 2 h with huperzine A (0.1-10 microM) improved neuronal survival. Huperzine A at a concentration of 1 microM significantly attenuated apoptosis by inhibiting the mitochondria-caspase pathway directly and indirectly.
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