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Related Experiment Videos

Resveratrol induces apoptosis in LNCaP cells and requires hydroxyl groups to decrease viability in LNCaP and DU 145

Gary Z Morris1, Roy L Williams, Mark S Elliott

  • 1Department of Chemistry and Biochemistry, Old Dominion University, Norfolk, Virginia, USA.

The Prostate
|September 5, 2002
PubMed
Summary

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Resveratrol effectively reduces prostate cancer cell viability by inducing apoptosis, independent of hormone sensitivity. Its three hydroxyl groups are crucial for this toxic effect, highlighting a key mechanism in cancer research.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Prostate cancer remains a significant health concern, necessitating research into novel therapeutic agents.
  • Resveratrol, a natural polyphenol, has demonstrated potential anti-cancer properties.
  • Understanding the mechanisms of resveratrol's action, including its structural requirements, is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the impact of resveratrol on prostate cancer cell viability.
  • To determine if resveratrol induces apoptosis in cancer cells.
  • To ascertain the role of resveratrol's hydroxyl groups in its cytotoxic effects.

Main Methods:

  • Utilized hormone-sensitive (LNCaP) and hormone-insensitive (DU 145) prostate cancer cell lines.

Related Experiment Videos

  • Treated cells with resveratrol, a derivative lacking hydroxyl groups (tri-methoxy-resveratrol), and diethylstilbestrol (DES).
  • Assessed cell viability using MTS assay and apoptosis via morphological changes, annexin V-FITC staining, and caspase activation.

    • Main Results:

    • Resveratrol and DES reduced viability in LNCaP cells.
    • Resveratrol induced apoptotic morphology, annexin V-FITC positivity, and caspase activation in LNCaP cells.
    • Tri-methoxy-resveratrol showed no effect on DU 145 cells and reduced toxicity in LNCaP cells compared to resveratrol.

    Conclusions:

    • Resveratrol exhibits toxicity towards prostate cancer cells irrespective of their hormone-responsive status.
    • The hydroxyl groups present on resveratrol are essential for its observed cell toxicity.
    • Resveratrol, unlike DES, triggers apoptosis through caspase-mediated pathways.