Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Digital in-line holography of microspheres.

W Xu1, M H Jericho, I A Meinertzhagen

  • 1Department of Physics, Dalhousie University, Halifax, Nova Scotia, Canada.

Applied Optics
|September 5, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Patterns of Bromodeoxyuridine Incorporation in the Nervous System of a Larval Ascidian, Ciona intestinalis.

The Biological bulletin·2018
Same author

Field evaporation of insulators and semiconductors: Theoretical insights for ZnO.

Ultramicroscopy·2015
Same author

Disintegration and field evaporation of thiolate polymers in high electric fields.

Ultramicroscopy·2015
Same author

Do dielectric nanostructures turn metallic in high-electric dc fields?

Nano letters·2014
Same author

Disintegration of graphene nanoribbons in large electrostatic fields.

Physical chemistry chemical physics : PCCP·2014
Same author

Slowly contracting muscles power the rapid jumping of planthopper insects (Hemiptera, Issidae).

Cell and tissue research·2013

Digital in-line holography (DIH) achieves submicrometer resolution for imaging particles. This technique accurately tracks particle positions in 3D, offering a powerful new tool for scientific research.

Area of Science:

  • Optics and Photonics
  • Materials Science
  • Biophysics

Background:

  • Accurate 3D particle tracking is crucial in various scientific fields.
  • Traditional imaging methods face limitations in resolution and throughput for microparticle analysis.

Purpose of the Study:

  • To investigate the capability of digital in-line holography (DIH) for high-resolution 3D particle imaging.
  • To determine the conditions for achieving submicrometer resolution in holographic reconstructions.
  • To evaluate DIH as a tool for 3D particle tracking.

Main Methods:

  • Utilized digital in-line holography (DIH) with numerical reconstruction.
  • Imaged micrometer-sized latex and ferrimagnetic beads in gelatin.
  • Analyzed holographic reconstructions to assess resolution and positional accuracy.

Related Experiment Videos

  • Employed intensity profile analysis and polynomial fitting for coordinate determination.

    • Main Results:

    • Achieved submicrometer resolution, limited by the wavelength of light.
    • Determined particle 3D coordinates with accuracy up to +/-50 nm.
    • Demonstrated imaging of both transparent and opaque particles.
    • Showcased DIH's potential for analyzing large particle collections.

    Conclusions:

    • DIH provides a powerful method for high-resolution 3D particle imaging and tracking.
    • The technique offers nanometer-level positional accuracy for microparticles.
    • DIH is a promising tool for applications requiring precise 3D particle analysis.