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Updated: Aug 17, 2026
Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
Published on: October 1, 2015
[Immunohistochemical detection of hepatitis C virus antigen from hepatocellular carcinoma]
1Institute of Viral Heaptitis, First Affiliated Hospital, WCUMS, Chengdu 610041.
Insights
Hepatitis C virus (HCV) infection is present in some liver cancer (HCC) cases in China. This suggests HCV may be a viral risk factor for HCC, alongside hepatitis B virus (HBV).
Area of Science:
- Hepatology
- Virology
- Oncology
Context:
- Hepatocellular carcinoma (HCC) is a major global health concern.
- Hepatitis B virus (HBV) is a known risk factor for HCC.
- The role of Hepatitis C virus (HCV) in HCC development in China requires further investigation.
Purpose:
- To investigate the prevalence of HCV infection and HCV antigen (HCAg) expression in HCC tissues.
- To simultaneously detect HCAg and Hepatitis B virus surface antigen (HBsAg) in HCC samples.
- To assess the potential role of HCV as a viral risk factor in HCC genesis.
Summary:
- Immunohistochemistry was used to analyze 40 HCC tumor tissues.
- HCV antigen (HCAg) was detected in 17.5% of cases, while HBsAg was found in 70%.
- Co-infection of HBsAg and HCAg was observed in 15% of patients, with a small percentage showing HCAg positivity alone.
Impact:
- This study indicates that HCV infection occurs in a subset of HCC patients in China.
- HCV may represent an additional viral risk factor for HCC development.
- Findings contribute to understanding the multifactorial etiology of HCC in the Chinese population.
Abstract:
To investigate the infection of HCV and the expression of HCV antigen (HCAg) in HCC, immunohistochemical protocol was performed on tumorous liver tissues from 40 patients with HCC to detect HCAg and hepatitis B virus surface antigen (HBsAg) simultaneously. The results showed that the positive rates for HCAg and HBsAg were 17.5% (7/40) and 70% (28/40) respectively. HBsAg and HCAg were simultaneously detected in six of 40 cases and only one case had HCAg positive alone in the liver tumorous tissue. The positive signals were localized in diffuse cytoplasm of hepatocytes and the positively stained cells were mainly distributed in local pattern. The results suggested that HCV infection did exist in some cases of HCC, so HCV might be a viral risk factor in addition to HBV in the genesis of HCC in China.
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