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Related Experiment Videos

[Studies on quality standards for Pollen Typhae(puhuang)].

S H Zhang1, S Q Wang, C H Liu

  • 1Hunan Academy of Traditional Chinese Medicine and Materia Medica, Changsha 410006, Hunan, China.

Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica
|September 6, 2002
PubMed
Summary

Quality standards for Pollen Typhae (Puhuang) were established using sensitive and specific analytical methods. This ensures reliable quality control for this important traditional Chinese medicine.

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Area of Science:

  • Pharmacognosy
  • Analytical Chemistry
  • Traditional Chinese Medicine

Background:

  • Pollen Typhae (Puhuang) is a widely used herb in Traditional Chinese Medicine.
  • Establishing robust quality standards is crucial for ensuring its therapeutic efficacy and safety.

Purpose of the Study:

  • To develop and validate quality standards for Pollen Typhae.
  • To identify key chemical markers for quality assessment.

Main Methods:

  • Thin-Layer Chromatography (TLC) was employed for the qualitative identification of key compounds.
  • High-Performance Liquid Chromatography (HPLC) was used for the quantitative determination of active components.

Main Results:

  • The analytical method demonstrated high sensitivity and specificity for identifying isorhamnetin-3-O-neohesperidoside, typhaneoside, isorhamnetin, and beta-sitosterol.

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  • HPLC analysis provided accurate quantification with excellent average recovery rates (97.77% for isorhamnetin-3-O-neohesperidoside and 98.42% for typhaneoside).

    • Conclusions:

    • The developed analytical method serves as a reliable standard for both qualitative and quantitative quality control of Pollen Typhae.
    • These findings contribute to the standardization and quality assurance of Puhuang in the pharmaceutical market.