Related Experiment Video
Updated: Sep 29, 2026

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment
Published on: November 23, 2017
[Effect of thrombin on cell proliferation and plasminogen activator inhibitor type (PAI-1) expression in human
1Department of Pediatrics, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
Objective:
To explore the effect of thrombin on cell proliferation and plasminogen-activator inhibitor Type 1(PAI-1) expression in human embryonic glomerular mesangial cells.
Methods:
Methyl-tetragolium (MTT) incorporation, fibrin plate assay and Northern blotting hybridization were used to examine the mesangial cells proliferation, protein activity and mRNA expression of PAI-1 in cultured human embryonic mesangial cells induced by thrombin, respectively.
Results:
Thrombin enhanced glomerular mesangial cell proliferation, PAI-1 protein activity and mRNA expression in a dose-dependent manner, which could be blocked by hirudin, a specific inhibitor of thrombin.
Conclusion:
Thrombin could enhance mesangial cell proliferation, and up-regulate protein activity and mRNA expression of PAI-1 in cultured human embryonic glomerular mesangial cells. It may play an important role in the pathogenesis of glomerulosclerosis induced by thrombin.
More Related Videos
Related Concept Videos
Intracellular Signaling Affects Focal Adhesions
Some...
Clot Retraction and Fibrinolysis

