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[Rapid high performance liquid chromatography for determination of phenylalanine and tyrosine in serum]
1Department of Clinical Laboratory, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
Objective:
To develop a rapid assay for the determination of phenylalanine and tyrosine in serum.
Methods:
The serum sample was injected directly into High performance liquid chromatography(HPLC) system after precipitation of protein with perchloric acid, and monitored at 190 nm with an ultraviolet detector.
Results:
Good linearity was observed in 6-1,512 mumol.L-1 range for phenylalanine, the intra-run and inter-run imprecisions(CV) were 3.68% and 4.01%, the recovery rate was 98.6%; good linearity was observed in 5.4-1,380.0 mumol.L-1 range for tyrosine, the intra-run and inter-run imprecisions were 1.87% and 2.59%, the average recovery rate was 98.9%.
Conclusion:
This method is simple, rapid, sensitive, accurate, and suitable for clinical application.
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