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Capillary electrophoretic enzyme immunoassay with electrochemical detection for cortisol
Min Jia1, Zhihui He, Wenrui Jin
1School of Chemistry and Chemical Engineering, and State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.
Journal of Chromatography. A
|September 7, 2002
Summary
A new capillary electrophoresis method enables sensitive cortisol detection using an enzyme immunoassay with electrochemical detection. This technique achieves a low limit of detection for accurate cortisol measurements.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Cortisol determination is crucial for diagnosing various endocrine disorders.
- Existing methods for cortisol analysis may lack sensitivity or require complex procedures.
Purpose of the Study:
- To develop a novel capillary electrophoretic enzyme immunoassay (CE-EIA) for sensitive cortisol determination.
- To establish an electrochemical detection method for enhanced accuracy.
Main Methods:
- A competitive enzyme immunoassay format was employed.
- Free and bound enzyme-labeled cortisol were separated using capillary electrophoresis.
- Electrochemical detection of the enzymatic reaction product (TMB(Ox)) was performed using a carbon fiber microdisk bundle electrode.
Main Results:
- The developed CE-EIA method achieved a concentration limit of detection (LOD) of 1.7 x 10(-1) mol/l.
- A mass LOD of 7.8 amol was obtained with a relative standard deviation of 3.3%.
- The method demonstrated reliability through verification with cortisol controls.
Conclusions:
- The novel CE-EIA with electrochemical detection offers a sensitive and reliable method for cortisol analysis.
- This technique has potential applications in clinical diagnostics and biochemical research.