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Volatile components from Anthriscus sylvestris (L.) Hoffm
Rein Bos1, Albert Koulman, Herman J Woerdenbag
1Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, Groningen University Institute for Drug Exploration, The Netherlands. r.bos@farm.rug.nl
Journal of Chromatography. A
|September 7, 2002
Summary
The study analyzed volatile compounds in Anthriscus sylvestris leaves and roots. Hydrodistillation revealed beta-phellandrene as the main component, with significant losses in dried samples.
Area of Science:
- Phytochemistry
- Natural Product Chemistry
- Analytical Chemistry
Background:
- Anthriscus sylvestris (L.) Hoffm., commonly known as wild beaked parsley, is a plant species with potential applications.
- Understanding the volatile composition of its different parts (leaves and roots) is crucial for exploring its uses.
Purpose of the Study:
- To identify and quantify the volatile components of fresh Anthriscus sylvestris leaves and roots.
- To compare the efficacy of hydrodistillation versus dichloromethane extraction for volatile compound analysis.
- To assess the impact of drying methods (air drying, freeze drying) on volatile constituent preservation.
Main Methods:
- Hydrodistillation of fresh Anthriscus sylvestris leaves and roots.
- Extraction using dichloromethane for fresh and dried leaf and root material.
- Analysis of volatile components using Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS).
Main Results:
- Monoterpenes constituted the dominant fraction (69-70%) in both leaf and root oils.
- Beta-phellandrene (39-45%) was the primary component in both leaf and root oils.
- Other significant components included beta-myrcene, sabinene, Z-beta-ocimene, and alpha-pinene.
- Dichloromethane extracts contained similar constituents but in much lower percentages compared to hydrodistillation.
- Drying processes (air and freeze drying) led to a significant loss of volatile compounds.
Conclusions:
- Hydrodistillation is more effective than dichloromethane extraction for analyzing volatile compounds in Anthriscus sylvestris.
- Fresh plant material yields a richer volatile profile compared to dried material.
- Drying significantly diminishes the volatile content, impacting potential applications of Anthriscus sylvestris extracts.