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Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
Published on: September 9, 2013
Sequencing Analysis of Oligodeoxynucleotide by Matrix-assisted Laser Desorption Ionization Time-of-flight Mass
Qing-Jin Yan1, Song-Cheng Yang, Yun Cai
1National Center of Biomedical Analysis, Beijing 100850, China.
Abstract:
A mixed matrix alpha-cyano 4-hydroxycinnamic acid (alpha-Cyano)/3-hydroxypicolinic acid (3HPA) was found to be a good matrix for the analysis of oligodeoxynucleotides by matrix-assisted laser desorption ionization time-of-flight mass spectrometry ( MALDI-TOF-MS). Using the mixed matrix, a similar sensitivity was obtained for the different oligodeoxynucleotides: d(T)(10), d(A)(10) and d(C)(10). This methodology can be used in combination with the partial digestion of oligodeoxynucleotides by 5'- and 3'-exonucleases as a powerful tool for sequencing analysis of oligonucleotides.
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