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Expression of Anti-PCNA mRNA Ribozyme by T7 Vaccinia Virus System in HeLa Cells
Zhe-Yuan Jin1, Guo-Rong Qi, Chang-De Lu
1Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
Abstract:
We have designed and synthesized a hammer-head Rz targeted to human proliferating cell nuclear antigen (PCNA) mRNA at site No.524. Results showed that Rz524 can cleave the transcribed substrate RNA site-specifically in vitro. A self trimming expression plasmid of this Rz was constructed and co-introduced into HeLa cells with T7 vaccinia virus. Dot-blotting hybridization showed that the Rz was expressed in HeLa cells.
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