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A test for antioxidant activity in cosmetic formulations
E Pelle1, T Mammone, K Marenus
1Estee Lauder Research Laboratories, 125 Pinelawn Road, Melville, NY 11747, USA.
Journal of Cosmetic Science
|September 10, 2002
Summary
This study developed a new method to measure antioxidant activity in cosmetic products. The adapted Randox assay allows for accurate assessment of total antioxidant status in finished formulations.
Area of Science:
- Cosmetic Science
- Analytical Chemistry
Background:
- Assessing antioxidant activity is crucial for cosmetic product development.
- Existing methods may not be suitable for analyzing finished formulations.
Purpose of the Study:
- To develop and validate a technique for quantifying antioxidant activity in finished cosmetic products.
- To adapt the Randox Assay for Total Antioxidant Status for use with cosmetic formulations.
Main Methods:
- Adapted the Randox Assay for Total Antioxidant Status kit.
- Utilized kinetic and end-point determinations for diluted samples.
- Evaluated antioxidant activity in a finished cosmetic product.
Main Results:
- The developed technique successfully quantified antioxidant activity.
- The finished product exhibited an IC(50) of 0.07 gm.
- A relative antioxidant activity concentration of 52.7 nmoles/mg was determined.
Conclusions:
- The adapted Randox assay provides a reliable method for determining antioxidant status in finished cosmetics.
- This technique facilitates quality control and product development in the cosmetic industry.
