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A novel phospholipase A(2) from the venom glands of Bungarus candidus: cloning and sequence-comparison
Inn-Ho Tsai1, Hwa-Yao Hsu, Ying-Ming Wang
1Institute of Biological Chemistry, Academia Sinica, and College of Sciences, National Taiwan University, PO Box 23-106, 106, Taipei, Taiwan, ROC. bc201@gate.sinica.edu.tw
Abstract:
The presence of phospholipase A(2) (PLA(2)) in the venom of Malayan krait (Bungarus candidus) and its structure were studied. The PLA(2) cDNAs from the venom gland of B. candidus (Indonesia origin) were amplified by the polymerase chain reactions (PCR) and cloned. The primers used were based on the cDNA sequences of several homologous B. multicinctus venom PLA(2)s. In addition to the A-chains of beta-bungarotoxins, a novel B. candidus PLA(2) was cloned and its full amino acid sequence deduced. Having totally 125 amino acid residues, the PLA(2) contains a pancreatic loop and is 61% identical to the acidic PLA(2) of king cobra venom. However, the enzyme was not detected from the venom sample. Its structural relationships to other elapid venom PLA(2)s were analyzed with a phylogenetic tree and discussed.