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Updated: Aug 22, 2026

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells
11:11

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

Published on: April 25, 2018

Characteristics of messenger ribonucleoprotein and putative messenger RNA from Ehrlich ascites tumor cells

G S Nakai

    Biomedicine / [Publiee Pour L'A.A.I.C.I.G.]
    |April 30, 1975
    PubMed

    Insights

    Researchers isolated messenger ribonucleoprotein (mRNP) and messenger RNA (mRNA) from Ehrlich ascites tumor cells. The study characterized the sedimentation properties and molecular weight of these crucial cellular components.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Background:

    • Messenger ribonucleoprotein (mRNP) complexes are essential for gene expression.
    • Understanding mRNA structure and stability is key to cellular function.

    Purpose of the Study:

    • To isolate and characterize mRNP and mRNA from Ehrlich ascites tumor cells.
    • To determine the sedimentation properties and molecular weight of isolated mRNA.

    Main Methods:

    • Isolation of mRNP and mRNA under conditions preventing degradation.
    • Dissociation of mRNP from polysomes using puromycin and EDTA.
    • Release of mRNA using proteinase K.
    • Sedimentation analysis (Svedberg units).

    Main Results:

    • mRNP dissociated from polysomes sedimented to 185S.
    • Putative mRNA released by proteinase K sedimented between 50S and 97S.
    • Maximum molecular weight of mRNA approximated 6-25 x 10^6 daltons.

    Conclusions:

    • The study successfully isolated and characterized tumor cell mRNP and mRNA.
    • The findings provide insights into the size and properties of mRNA in these cells.

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