Related Experiment Videos
[Transient expression of fusion protein with a chimeric HBsAg-HSP70 construct in HepG2 cells]
Zeng Wei Liang1, Ying Hua Lan, Hong Ren
1Institute for Viral Hepatitis, Chongqing University of Medical Science, Chongqing 400010, China.
Objective:
To investigate transient expression of fusion protein with a chimeric HBsAg-HSP70 construct in HepG2 cells.
Methods:
Enkaryotic expression plasmids inserted HBsAg gene or chimeric HBsAg-HSP70 gene were prepared and transfected into HepG2 cells by means of cationic liposome. mRNA were detected by RT-PCR and proteins expressed in the cells were detected by immunocytochemistry 48 hours later. HBsAg in cultured supernatants and cell lysates were assayed by ELISA.
Results:
Fusion protein (HBsAg-HSP70) transient expression in HepG2 cells were confirmed by RT-PCR, immunocytochemistry or ELISA, but fusion protein was not assayed in cell cultured supernatants by ELISA.
Conclusions:
Transfection of HepG2 cells with a chimeric HBsAg-HSP70 construct leads to express fusion protein, but it does not secrete into cell cultured supernatants.