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[Epitope mapping of hepatitis C virus non-structure protein 5 from a 7 peptide phage library by using immobilized
Yan Wei Zhong1, Jun Cheng, Gang Wang
1Gene Therapy Research Center, Institute of infectious Diseases, 302th Hospital of PLA, Beijing 100039, China.
Abstract
Objective:
To screen HCV NS5 mimotopes by using monoclonal antibody and phage peptide library.
Methods:
By using HCV NS5 monoclonal antibody as selective molecule, a 7 peptide phage library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.
Results:
Twelve positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was confirmed as the mimotope of HCV NS5.
Conclusions:
HCV mimotope is obtained by phage peptide library screening. The result provides a new approach for HCV therapy and vaccine development.