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Zymographic detection of cinnamic acid decarboxylase activity
Núria Prim1, F I Javier Pastor, Pilar Diaz
1Department of Microbiology, Faculty of Biology, University of Barcelona, Avenue Diagonal 645, 08028 Barcelona, Spain.
Journal of Microbiological Methods
|September 12, 2002
Abstract:
The manuscript includes a concise description of a new, fast and simple method for detection of cinnamic acid decarboxylase activity. The method is based on a color shift caused a by pH change and may be an excellent procedure for large screenings of samples from natural sources, as it involves no complex sample processing or purification. The method developed can be used in preliminary approaches to biotransformation processes involving detection of hydroxycinnamic acid decarboxylase activity.