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Related Experiment Videos

Regulatory volume decrease in human esophageal epithelial cells.

Geraldine S Orlando1, Nelia A Tobey, Paul Wang

  • 1Department of Medicine, Tulane University School of Medicine and Veterans Administration Medical Center, New Orleans, Louisiana 70112 - 2699, USA. gorlando@tulane.edu

American Journal of Physiology. Gastrointestinal and Liver Physiology
|September 12, 2002
PubMed
Summary

Human esophageal cells protect against hyposmolal stress via regulatory volume decrease (RVD). This mechanism, involving ion channels and transporters, functions even in acidic conditions, offering protection in reflux disease.

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Area of Science:

  • Cell biology
  • Physiology
  • Gastroenterology

Background:

  • Human esophageal epithelial cells face regular hyposmolal stress.
  • Destructive effects occur when hyposmolal solutions contact basolateral membranes after barrier breach.

Purpose of the Study:

  • Examine hyposmolal stress effects on esophageal epithelial cells.
  • Model used: HET-1A cell line.

Main Methods:

  • Cell volume and hyposmolal stress response assessed using Coulter Counter Multisizer II.
  • Pharmacologic agents and ion substitutions employed to study RVD mechanisms.

Main Results:

  • HET-1A cells acted as osmometers, swelling up to 15-20% under hyposmolal stress (118 mosmol/kg H2O).

Related Experiment Videos

  • Cells demonstrated regulatory volume decrease (RVD), restoring baseline volume within 30 minutes.
  • RVD involved K+ and Cl- conductances, followed by KCl cotransporter activation.
  • Acidic environment (pH 6.6) partially inhibited RVD.

    • Conclusions:

    • Human esophageal epithelial cells possess RVD mechanisms to counteract hyposmolal stress.
    • Redundant RVD pathways offer protection, potentially beneficial in acidic reflux conditions.