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A highly sensitive assay for proteases using staphylococcal protein A fused with enhanced green fluorescent protein

Hiroyoshi Fujino1, Takashi Aoki, Hiroyuki Watabe

  • 1Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan.

Bioscience, Biotechnology, and Biochemistry
|September 13, 2002
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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...