Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peeling process in living cell movement under shear flow.

Emmanuel Décavé1, Daniel Garrivier, Yves Bréchet

  • 1CEA/Grenoble, DRFMC/SI3M, 17 rue des Martyrs, 38054 Grenoble cedex 9, France.

Physical Review Letters
|September 13, 2002
PubMed
Summary

Cell membranes peel from substrates under shear flow, with peeling velocity linked to applied force and adhesion bridges. Dissipation is calculated using out-of-equilibrium thermodynamics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fluorescence Quantification of Silicone Oil Release upon Contact with Liquid Therapeutic Formulations.

Methods and protocols·2026
Same author

Fast and precise magnetophoresis of superparamagnetic nanoparticles on a micro-magnetic substrate in a static liquid environment.

Lab on a chip·2026
Same author

Unveiling the Interactions between a Protein Derived from Barnacle Adhesive and Surfaces Using Surface Plasmon Resonance Imaging.

ACS applied bio materials·2026
Same author

Simulating mono- and multiprotein phosphorylation within nanoclusters.

Physical review. E·2026
Same author

Magnetically Localized Detection of Amplified DNA Using Biotinylated and Fluorescent Primers and Magnetic Nanoparticles.

Biosensors·2025
Same author

Evaluating surfactant effectiveness in preventing antibody adsorption directly on medical surfaces using a novel device.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2024

Area of Science:

  • Cellular mechanics
  • Biophysics
  • Adhesion science

Background:

  • Understanding cell-substrate interactions is crucial for cell migration and tissue engineering.
  • The behavior of the cell membrane under external forces is not fully understood.

Purpose of the Study:

  • To directly observe and analyze the contact area dynamics between Dictyostelium discoideum cells and a solid substrate under shear flow.
  • To investigate the relationship between peeling velocity and applied force.
  • To explain the observed phenomena using the behavior of individual adhesion bridges and thermodynamic principles.

Main Methods:

  • Direct optical observation of cell-substrate contact area.
  • Application of controlled shear flow.
  • Experimental measurement of peeling velocity and applied force.

Related Experiment Videos

  • Analysis based on adhesion bridge behavior and out-of-equilibrium thermodynamics.

    • Main Results:

    • Demonstrated that the cell membrane peels off the substrate under shear flow.
    • Established an experimental relationship between peeling velocity and applied force.
    • Explained the peeling mechanism through the behavior of individual adhesion bridges.
    • Quantified the dissipation during the peeling process.

    Conclusions:

    • Cell membrane peeling is a key mechanism in cell-substrate detachment under shear.
    • Adhesion bridge dynamics govern the force-velocity relationship during peeling.
    • Out-of-equilibrium thermodynamics provides a framework for understanding energy dissipation in this process.