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Polyuronides in Avocado (Persea americana) and Tomato (Lycopersicon esculentum) Fruits Exhibit Markedly Different Patterns of Molecular Weight Downshifts during Ripening.

Plant physiology·1993
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Updated: Jun 24, 2026

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates
10:46

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates

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Alterations in Structural Polysaccharides during Liquefaction of Tomato Locule Tissue.

G. W. Cheng1, D. J. Huber

  • 1Horticultural Sciences Department, P.O. Box 110690, University of Florida, Gainesville, Florida 32611.

Plant Physiology
|June 1, 1996
PubMed
Summary
This summary is machine-generated.

Tomato locule liquefaction involves changes in pectin and hemicellulose. High molecular mass pectins, rich in galactose, arabinose, and xylose, are key to fruit softening during ripening.

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Last Updated: Jun 24, 2026

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Area of Science:

  • Plant Biochemistry
  • Fruit Ripening Physiology
  • Molecular Plant Science

Background:

  • Tomato locule tissue undergoes significant liquefaction during ripening.
  • Understanding the molecular changes in cell wall polysaccharides is crucial for explaining fruit softening.

Purpose of the Study:

  • To investigate the solubility, molecular mass, and glycosyl composition of tomato locule pectic and alkali-soluble polysaccharides.
  • To identify specific polysaccharide features contributing to the unique properties and liquefaction of tomato locule tissue.

Main Methods:

  • Preparation of ethanol-insoluble solids from de-seeded locule tissue at immature green, mature green, and breaker stages.
  • Sequential extraction of pectins using water, chelator (trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid), mild alkali (Na2CO3), and strong alkali (KOH).
  • Analysis of polysaccharide solubility, molecular mass, and glycosyl composition.

Main Results:

  • Nearly 85% of locule pectins were soluble in mild extraction solutions (water, chelator, Na2CO3) at the immature green stage, with solubility increasing slightly during development.
  • Noncovalently associated polymers maintained high molecular mass throughout liquefaction, while alkali-extracted polymers had lower molecular mass.
  • Locule pectins were rich in galactose, arabinose, and xylose, with similar deglycosylation trends during ripening. Hemicelluloses were rich in glucose, xylose, and arabinose, showing molecular mass downshifts with minimal compositional changes.

Conclusions:

  • High molecular mass, noncovalently associated pectins, rich in specific sugars, are characteristic of immature tomato locules.
  • Both pectin and hemicellulose undergo molecular mass reduction during ripening-associated liquefaction.
  • Xylose-rich acidic polymers were also identified in the KOH-soluble fraction, distinct from noncovalently associated pectins.