Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Representational difference analysis between Afa/Dr diffusely adhering Escherichia coli and nonpathogenic E. coli

Anne-Beatrice Blanc-Potard1, Colin Tinsley, Isabel Scaletsky

  • 1Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 510, Faculté de Pharmacie Paris XI, 92296 Châtenay-Malabry, France.

Infection and Immunity
|September 14, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neisseria meningitidis filamentous phage MDA promotes colonisation by selecting hyperadhesive pili variants.

Nature communications·2025
Same author

Erratum: Modeling Persistent Pseudomonas aeruginosa Infection in Wounded Zebrafish Larvae.

Journal of visualized experiments : JoVE·2025
Same author

Modeling Pseudomonas aeruginosa-Staphylococcus aureus interactions in zebrafish to assess the host inflammatory response upon co-infection.

Scientific reports·2025
Same author

Structure of the virulence-associated <i>Neisseria meningitidis</i> filamentous bacteriophage MDAΦ.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Intracellular Pseudomonas aeruginosa persist and evade antibiotic treatment in a wound infection model.

PLoS pathogens·2025
Same author

The hypervirulent Group B Streptococcus HvgA adhesin promotes central nervous system invasion through transcellular crossing of the choroid plexus.

Fluids and barriers of the CNS·2024

This study identified unique DNA sequences in diarrheagenic Escherichia coli (E. coli) strains. Three specific sequences were found to be highly prevalent in Afa/Dr DAEC strains, suggesting their role in virulence.

Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • Diffusely adhering E. coli (DAEC) strains with Afa/Dr adhesins are linked to diarrhea and urinary tract infections (UTIs).
  • Molecular characterization of these pathogens is crucial for understanding their virulence mechanisms.

Purpose of the Study:

  • To conduct an extensive molecular investigation of an Afa/Dr DAEC strain (C1845) using representational difference analysis.
  • To identify DNA sequences specific to the pathogenic strain C1845 and absent in the nonpathogenic K-12 strain.

Main Methods:

  • Representational difference analysis (RDA) was employed to compare the genomes of E. coli strains C1845 and K-12.
  • The distribution of identified unique sequences was analyzed across various clinical isolates and reference strains.

Related Experiment Videos

Main Results:

  • Strain C1845 contains a pathogenicity island (PAI(CFT073)) and iron transport systems, but lacks known host-subverting factors like type III secretion systems.
  • Three novel DNA sequences (MO30, S109, S111) were identified as specific to strain C1845.
  • These sequences showed high prevalence (77-80%) in Afa/Dr strains and low prevalence (12-23%) in non-Afa/Dr strains.
  • Strain IH11128, an Afa/Dr DAEC UTI isolate, was found to be genetically similar to C1845.

Conclusions:

  • The identified unique sequences (MO30, S109, S111) are potential virulence factors associated with Afa/Dr DAEC.
  • These findings contribute to understanding the genetic basis of pathogenicity in DAEC strains.
  • Afa/Dr DAEC strains causing UTIs may be closely related to those causing diarrhea.