Related Experiment Video
Updated: Sep 29, 2026

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis
Published on: June 4, 2021
Putrescine Channels in the Plasma Membrane of Arabidopsis thaliana
L. Giromini1, A. Paina, R. Cerana
1Dipartimento di Biologia, Universita degli Studi di Milano, via Celoria, 26, I-20133 Milano, Italy.
Abstract:
The patch-clamp technique was used in the whole-cell configuration to study plasma membrane channels permeable to the diamine putrescine in protoplasts isolated from cultured cells of Arabidopsis thaliana L. Under our experimental conditions, no channels selectively mediating putrescine influx were observed. Inward K+ channels showed a low permeability to putrescine, the permeability ratio of putrescine relative to K+ being around 0.1. Further characterization of the previously identified outward channels mediating putrescine efflux (R. Colombo, R. Cerana, N. Bagni [1992] Biochem Biophys Res Commun 182: 1187-1192) indicated that their activity was regulated by the overall ion concentration of the external medium.
Related Concept Videos
Cell Signaling in Plants
Protein Transport to the Inner Chloroplast Membrane
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Protein Transport to the Outer Chloroplast Membrane
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
Regulation of Transpiration by Stomata

