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Updated: Aug 5, 2026
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An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Published on: September 15, 2015
Isolation, purification and preliminary characterization of cryophilic proteases of marine origin
M H Salamanca1, C Barría, J A Asenjo
1Center for Biochemical Engineering and Biotechnology, Department of Chemical Engineering, University of Chile, Santiago.
Bioseparation
|September 18, 2002
Abstract:
The isolation and preliminary characterization of a trypsin-like protease with high activity at 20 degrees C is described. This protease was isolated from Antarctic krill (Euphasia superba) by a two-step chromatography process and the use of zymogram analyses. The protease has a molecular weight of 30 kDa and a pI of 4.1. Its specific activity at 20 degrees C on BAPNA is 0.5 U/mg.
