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Historical perspectives on lung edema clearance.
Edward D Crandall1, Richard M Effros
1Will Rogers Institute Pulmonary Research Center, University of Southern California, Los Angeles, California 90033, USA. ecrandal@us.edu
Journal of Applied Physiology (Bethesda, Md. : 1985)
|September 18, 2002
Summary
Early research on pulmonary epithelial fluid transport used intact lungs. While limited in cell identification, these models are crucial for validating in vitro findings and understanding clinical relevance in lung physiology.
Area of Science:
- Pulmonary Physiology
- Epithelial Transport
- Respiratory Medicine
Background:
- Early investigations of pulmonary epithelial fluid transport relied on whole animal or isolated lung preparations.
- These methods, while not pinpointing specific cells, are vital for assessing the physiological and clinical significance of in vitro cell studies.
Purpose of the Study:
- To review methodologies for studying fluid transport across the pulmonary epithelium in intact lungs.
- To discuss the advantages and limitations of different approaches for examining vascular and air space exchange.
Main Methods:
- Review of three distinct experimental approaches used in intact lung preparations.
- Analysis of methods for studying fluid and solute exchange between blood and air spaces.
Main Results:
- Intact lung preparations are indispensable for validating in vitro findings.
- Different methods offer unique insights into pulmonary transport but have inherent limitations.
Conclusions:
- Despite limitations in cellular resolution, intact lung models remain essential for bridging in vitro discoveries with in vivo physiological and clinical realities.
- Understanding the advantages and drawbacks of various intact lung study methods is key for accurate interpretation of pulmonary transport research.