Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tripartite molecular beacons.

Razvan Nutiu1, Yingfu Li

  • 1Department of Biochemistry, McMaster University, Health Sciences Center, 1200 Main Street West, Hamilton, ON L8N 3Z5, Canada.

Nucleic Acids Research
|September 18, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LRP1 Activation Promotes Metabolic Reprogramming and Mrc1 Expression to Attenuate LPS-Induced Cognitive Deficits: An Integrated Omics Analysis.

CNS neuroscience & therapeutics·2026
Same author

A Portable Colorimetric Device for Rapid Bacterial Detection with Cleavable Functional Nucleic Acid Probes for A Common Bacterial Endoribonuclease.

Angewandte Chemie (International ed. in English)·2026
Same author

Engineering High-Performance Aptamers via Optimized SELEX and Structure-Guided Dimer Assembly for Clinical Malaria Diagnostics.

Analytical chemistry·2026
Same author

Metabolic syndrome and the risk of perioperative ischemic stroke in non-cardiac surgery: a case-control retrospective study.

Frontiers in endocrinology·2026
Same author

A general strategy to enhance aptamer affinity by suppressing dissociation through symmetric assembly.

Nucleic acids research·2026
Same author

Translating a miRNA Signal into Physical Immunomodulation via Programmed DNA Network Assembly on Mitochondria.

Journal of the American Chemical Society·2026
Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026
Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026
Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026
Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026
See all related articles

This study introduces tripartite molecular beacons (TMBs), a novel DNA probe design. TMBs offer a more affordable and flexible alternative to standard molecular beacons for nucleic acid detection.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Chemical Biology

Background:

  • Molecular beacons (MBs) are valuable fluorescent DNA probes for nucleic acid detection.
  • MBs are costly and lack flexibility due to probe synthesis, purification, and dual-end modifications.
  • Existing MBs limit fluorophore choice and surface immobilization options.

Purpose of the Study:

  • To develop a more affordable and flexible alternative to standard molecular beacons.
  • To introduce tripartite molecular beacons (TMBs) for nucleic acid detection.
  • To assess TMBs' efficacy and flexibility compared to conventional MBs.

Main Methods:

  • Design of tripartite molecular beacons (TMBs) using an unmodified oligonucleotide and two universal arms.
  • Attachment of a fluorophore and quencher to separate universal arms.

Related Experiment Videos

  • Evaluation of TMBs' performance in nucleic acid target signaling and single-nucleotide mismatch discrimination.

    • Main Results:

    • TMBs demonstrate comparable effectiveness to standard MBs in detecting nucleic acid targets.
    • TMBs accurately discriminate targets differing by a single nucleotide.
    • TMBs offer enhanced flexibility for fluorophore selection and surface immobilization.

    Conclusions:

    • Tripartite molecular beacons (TMBs) present a cost-effective and adaptable solution for nucleic acid detection.
    • TMBs overcome limitations of standard MBs regarding cost, flexibility, and immobilization.
    • The TMB design holds promise for broader applications in nucleic acid detection technologies.