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On-probe digestion of bacterial proteins for MALDI-MS
William A Harris1, James P Reilly
1Department of Chemistry, Indiana University, Bloomington 47405, USA.
Analytical Chemistry
|September 19, 2002
Abstract:
On-probe digestion combined with MALDI mass spectrometry is studied as a rapid method for the analysis and identification of bacterial proteins. The use of trypsin adsorbed to the probe surface reduces the digestion time from hours to minutes. A high amount of trypsin must be applied to the probe for the successful digestion of bacterial proteins. Mass spectra of the digest contain a number of low-mass digest fragments. Several components of a B. subtilis bacterial digest can be identified through postsource decay and database searching.