Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The SCL complex regulates c-kit expression in hematopoietic cells through functional interaction with Sp1.

Eric Lécuyer1, Sabine Herblot, Marianne Saint-Denis

  • 1Clinical Research Institute of Montreal and from the Departments of Pharmacology, Biochemistry, and Molecular Biology, Université de Montréal, Quebec, Canada.

Blood
|September 20, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Digesting detention: how to document eating patterns in jail.

BMC nutrition·2026
Same author

Soft tissue CBCT for diagnosing and planning treatment of altered passive eruption. A cross-sectional study.

The international journal of esthetic dentistry·2026
Same author

Real-world hospital pharmacy and nursing study assessing the time and motion comparison of 3 different marketed formulations of a cephalosporin antibiotic.

American journal of health-system pharmacy : AJHP : official journal of the American Society of Health-System Pharmacists·2026
Same author

Comparative induced pluripotent stem cell models of young, aged, and progeroid, as a resource to study human aging trajectories.

Stem cell research·2026
Same author

Socket Shield Technique for Single and Adjacent Sites.

Atlas of the oral and maxillofacial surgery clinics of North America·2026
Same author

Localized regulation of cell junction mRNAs is required for epithelial cell integrity.

RNA (New York, N.Y.)·2026

Stem cell leukemia (SCL) protein directly regulates c-Kit expression by forming a complex with other factors. This interaction is crucial for hematopoietic cell fate determination and differentiation.

Area of Science:

  • Hematopoiesis
  • Molecular Biology
  • Gene Regulation

Background:

  • Hematopoietic cell fate is determined by transcription factor interactions.
  • Stem cell leukemia (SCL), a bHLH factor, is vital for hematopoietic development.
  • SCL's target genes and mechanism of action require elucidation.

Purpose of the Study:

  • To identify SCL target genes and elucidate its molecular mechanism in hematopoietic development.
  • To investigate the coexpression and interaction of SCL and c-Kit in hematopoietic progenitors.
  • To define the role of SCL in regulating c-Kit transcription.

Main Methods:

  • Single-cell coexpression analysis of SCL and c-Kit.
  • Transgenic mouse models with ectopic SCL expression.

Related Experiment Videos

  • Transient transfection assays and coimmunoprecipitation.
  • Chromatin immunoprecipitation (ChIP) assays.

    • Main Results:

    • SCL and c-Kit are coexpressed in hematopoietic progenitors.
    • Ectopic SCL expression sustains c-Kit transcription in B lymphocytes.
    • SCL acts as a nucleation factor for a complex that enhances the c-Kit promoter.
    • The SCL complex, including LMO2, GATA-1/2, E2A, and Ldb-1, interacts with Sp1 to bind the c-Kit promoter.
    • SCL, E2A, and Sp1 co-occupy the c-Kit promoter in vivo.

    Conclusions:

    • c-Kit is a direct transcriptional target of the SCL complex.
    • Combinatorial interaction of SCL complex members is essential for c-Kit promoter activation.
    • Down-regulation of SCL inactivates the SCL complex, potentially driving hematopoietic cell differentiation.