Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evolutionary dynamics of viral attenuation.

Marty R Badgett1, Alexandra Auer, Leland E Carmichael

  • 1Section of Integrative Biology, Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712-1023, USA.

Journal of Virology
|September 20, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct evolutionary patterns of endemic and emerging parvoviruses and the origin of a new pandemic virus.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Modeling the Phage Properties Best for Therapy.

Viruses·2026
Same author

Modeling the phage properties best for therapy.

bioRxiv : the preprint server for biology·2026
Same author

Canine parvovirus infection affects host cell nucleolar organization and ribosome biogenesis.

Communications biology·2026
Same author

Short and long term suppression of host populations by novel pathogens.

bioRxiv : the preprint server for biology·2025
Same author

Distinct evolutionary patterns of endemic and emerging parvoviruses, and the origin of a new pandemic virus.

bioRxiv : the preprint server for biology·2025
Same journal

Magnolol inhibits porcine epidemic diarrhea virus infection by suppressing cathepsin L expression <i>in vitro</i> and <i>in vivo</i>.

Journal of virology·2026
Same journal

Dysregulation of miRNAs has broad impacts on virus infection in <i>Drosophila</i>.

Journal of virology·2026
Same journal

Identification of GRP78 as a novel host factor that facilitates zoonotic porcine deltacoronavirus internalization and replication via clathrin-mediated endocytosis.

Journal of virology·2026
Same journal

MGF110-2L deletion prevents IFN-I and inflammatory response, resulting in partial attenuation and protection against virulent ASFV.

Journal of virology·2026
Same journal

Periodic genome sequences facilitate packaging in a single-stranded DNA virus.

Journal of virology·2026
Same journal

A novel monoclonal antibody targeting the hemagglutinin-neuraminidase of peste des petits ruminants virus maintains neutralizing activity by blocking viral adsorption and receptor interaction.

Journal of virology·2026
See all related articles

This study tracked canine parvovirus (CPV) genetic changes over 115 passages, revealing viral evolution driven by recombination and selective mutation, leading to attenuation.

Area of Science:

  • Virology
  • Genetics
  • Molecular Biology

Background:

  • Canine parvovirus (CPV) causes severe disease in dogs.
  • Understanding viral attenuation is crucial for vaccine development and disease control.

Purpose of the Study:

  • To investigate the genetic alterations and evolutionary patterns of a CPV strain during serial passage in cell culture.
  • To identify mutations associated with viral attenuation.

Main Methods:

  • Serial passage of a CPV strain in dog kidney cells for 115 transfers.
  • Whole-genome sequencing of viral populations at multiple passage points (0, 3, 30, 50, 80, 115).
  • Analysis of genetic variation, fixation of mutations, and evidence of recombination.

Main Results:

Related Experiment Videos

  • Sixteen genetic changes were fixed by passage 115.
  • A significant reduction in genetic variation occurred after a plaque-cloning step at passage 112.
  • The evolutionary pattern suggested recombination rather than independent selection of mutations.
  • Thirteen observed changes were consistent with those found in other CPV or feline panleukopenia virus isolates.

Conclusions:

  • Viral attenuation in CPV is a complex process involving genetic recombination and selection.
  • Serial passage in cell culture can induce significant genetic changes, offering insights into viral evolution.
  • The identified genetic changes may inform the development of attenuated CPV vaccines.