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Updated: Jul 22, 2026

Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
Published on: October 30, 2015
Biphasic oxygen kinetics of cellular respiration and linear oxygen dependence of antimycin A inhibited oxygen
Eveline Hütter1, Kathrin Renner, Pidder Jansen-Dürr
1Institute for Biomedical Aging Research, Austrian Academy of Sciences, Innsbruck.
Abstract:
Oxygen kinetics in fibroblasts was biphasic. This was quantitatively explained by a major mitochondrial hyperbolic component in the low-oxygen range and a linear increase of rotenone- and antimycin A-inhibited oxygen consumption in the high-oxygen range. This suggests an increased production of reactive oxygen species and oxidative stress at elevated, air-level oxygen concentrations. The high oxygen affinity of mitochondrial respiration provides the basis for the maintenance of a high aerobic scope at physiological low-oxygen levels, whereas further pronounced depression of oxygen pressure induces energetic stress under hypoxia.
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