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Updated: Oct 9, 2026
Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells
Published on: October 20, 2023
Protocol for isolation and cryopreservation of human peripheral blood mononuclear cells for high-resolution
Alejandra Romero1, Alba Timón-Gómez2, Jaime R Willis2
1VASCage-Centre on Clinical Stroke Research, Innsbruck 6020, Austria.
Abstract:
High-resolution respirometry (HRR) of peripheral blood mononuclear cells (PBMCs) relies on controlled preanalytical procedures. Here, we present an optimized workflow for PBMC isolation from human whole blood, followed by short-term cryopreservation at -80°C. The protocol includes rapid thawing to preserve cell viability, washing steps to minimize platelet contamination, and quality-control assessment of PBMC number, viability, and composition before HRR measurements. Critical handling steps are optimized and validated by HRR against freshly prepared cells.
