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[Determination of gallic acid in garden burnet by HPLC]
Abstract
Objective:
To establish a HPLC method for the determination of gallic acid in garden burnet.
Method:
The sample was extracted with methanol under ultrasonic management for 25 min. Chromatographic conditions: Shimadzu LC-6A with SPD-6AV detector at 270 nm and auto injector SIL-6A, methanol-0.025 mol/L phosphoric acid(15:85) as the mobile phase. Shin-pack CLC-ODS column(6.0 mm x 15 mm).
Result:
Gallic acid in garden burnet was baseline separated and determined. The average content was 0.22%, recovery rate 97.4% and relative standard deviation 1.3%.
Conclusion:
Simple, rapid and accurate, the method provides new indexes for quality control of Garden Burnet.