Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Missing mass effect in biosensor's QCM applications.

M V Voinova1, M Jonson, B Kasemo

  • 1Department of Applied Physics, Chalmers University of Technology and Göteborg University, S-412 96, Göteborg, Sweden. voinova@fy.chalmers.se

Biosensors & Bioelectronics
|September 24, 2002
PubMed
Summary

Quartz Crystal Microbalance (QCM) in liquid applications shows deviations from ideal mass response due to viscoelasticity. A new theory provides an analog of the Sauerbrey relation for accurate biosensing in biofluids.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Magnetoconductance Anisotropies and Aharonov-Casher Phases.

Physical review letters·2022
Same author

Epidemiology of suicidal feelings in an ageing Swedish population: from old to very old age in the Gothenburg H70 Birth Cohort Studies.

Epidemiology and psychiatric sciences·2019
Same author

Ferritin-supported lipid bilayers for triggering the endothelial cell response.

Colloids and surfaces. B, Biointerfaces·2016
Same author

Spin-mediated Photomechanical Coupling of a Nanoelectromechanical Shuttle.

Physical review letters·2016
Same author

Rashba Splitting of Cooper Pairs.

Physical review letters·2016
Same author

Single-electron shuttle based on electron spin.

Physical review letters·2014

Area of Science:

  • Biophysics
  • Materials Science
  • Analytical Chemistry

Background:

  • Quartz Crystal Microbalance (QCM) enables in situ studies of biomaterials like proteins and cells in liquid.
  • Viscoelastic properties of adsorbed biomaterials cause deviations from the Sauerbrey relation, limiting biosensing accuracy.
  • Understanding these deviations is crucial for reliable QCM applications in biofluids.

Purpose of the Study:

  • To develop a rigorous theoretical analysis explaining the deviation from ideal mass response in QCM measurements of soft overlayers in liquid.
  • To derive an analog of the Sauerbrey relation applicable to layered viscous/viscoelastic media.
  • To enable accurate physical interpretation of QCM experimental data for surface mass measurements in biofluids.

Main Methods:

Related Experiment Videos

  • Theoretical analysis of QCM response for soft overlayers in contact with liquid.
  • Development of an analog of the Sauerbrey relation for layered viscous/viscoelastic media.
  • Comparison of theoretical predictions with experimental QCM data from supported membranes.

    • Main Results:

    • A rigorous theoretical framework explaining the deviation from the ideal Sauerbrey relation for soft overlayers in liquid was established.
    • An analog of the Sauerbrey relation for layered viscous/viscoelastic media was derived, allowing for correct interpretation of QCM data.
    • A novel physical effect termed 'missing mass' in liquid phase measurements was predicted.
    • Theoretical results were validated against experimental QCM measurements on supported membranes.

    Conclusions:

    • The developed theory accurately explains deviations in QCM measurements due to viscoelasticity in liquid.
    • The derived analog of the Sauerbrey relation is essential for precise surface mass determination of biomolecular films in biofluids.
    • The 'missing mass' effect highlights a critical consideration for interpreting QCM data in liquid phase biosensing.
    • This work enhances the reliability and applicability of QCM for in situ biosensing in complex biological media.