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[Histopathologic and ultrastructural study of tumors induced by murine sarcoma virus (msv) (author's transl)]
Abstract:
Various growth phases of Moloney murine sarcoma virus (M-MSV) induced tumors in suckling and young adult BALB/c mice have been studied by light and electron microscopy. In the early phase (3-6 days following M-MSV), observations at the injection site of the thigh muscles consisted of endo- and perimysial edema "activated" muscle satellite, cells, endothelial cells and fibroblasts, scattered type C virus particles within the muscle fibers, muscle fibers and endomysial cells undergoing necrosis and macrophage and granulocyte infiltration. During the overt tumor phase (6-12 days following M-MSV), observation of neoplastic tissue disclosed proliferation of several cell type (endothelial, periosteal, fibroblasts, etc.), poorly differentiated myoblasts along with atypical rhabdomyoblast-like cells and sarcolytes, type C virus budding from muscle fiber and myoblast plasma membrane, and intense degenerative and regenerative changes in the muscle fibers together with more profuse granulocyte infiltration. The regressive phase (13-21 days following M-MSV) presented reduced cellularity of the neoplastic tissue, a decrease in blast cells, diminishing granulocyte infiltration with contemporaneous appearance of prominent lymphocyte foci and gradual disappearance of virus particles. Although many cell types of mesenchymal origin proliferate following M-MSV infection, the above morphological findings indicate that striated muscle is a preferential site for virus replication and transformation. Furthermore, the peculiar virus cell relationships leading to cell lysis and continuous recruitment of newly infected cells have been widely documented. In the light of these findings it is suggested that, besides the host immune control of virus spread and tumor cell multiplication, the non clonal growth pattern of M-MSV induced tumors is a crucial factor in determining the spontaneous regression which occurs with high frequency in this experimental system.
Insights
Moloney murine sarcoma virus (M-MSV) causes tumors in mice, with striated muscle being a key site for virus replication and transformation. The non-clonal growth pattern of these M-MSV tumors is crucial for their spontaneous regression.
Area of Science:
- Virology
- Oncology
- Cell Biology
Context:
- Moloney murine sarcoma virus (M-MSV) induces tumors in mice.
- Tumorigenesis involves complex cellular and viral interactions.
- Understanding tumor growth dynamics is key to explaining regression.
Purpose:
- To investigate the morphological changes during the growth phases of M-MSV induced tumors.
- To identify the preferential site of M-MSV replication and transformation.
- To elucidate the factors contributing to the spontaneous regression of M-MSV tumors.
Summary:
- Microscopic analysis revealed distinct phases of M-MSV tumor development in BALB/c mice, from initial edema and cell infiltration to overt neoplastic proliferation and eventual regression.
- Striated muscle was identified as a preferential site for M-MSV replication and cellular transformation, characterized by viral budding and degenerative/regenerative changes.
- The study highlights that while immune responses play a role, the non-clonal growth pattern of M-MSV tumors is a critical factor in their high frequency of spontaneous regression.
Impact:
- Provides detailed morphological insights into virus-induced tumor development and regression.
- Identifies striated muscle as a primary target for M-MSV, informing future research on viral oncogenesis.
- Suggests that non-clonal growth dynamics are a significant factor in experimental tumor regression, potentially applicable to other viral tumor models.