Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular structural studies of human factor VIII.

P A McKee, J C Andersen, M E Switzer

    Annals of the New York Academy of Sciences
    |January 20, 1975
    PubMed
    Summary

    Human factor VIII, a large glycoprotein, comprises identical 195,000-molecular-weight subunits. This molecule is responsible for both antihemophilic and von Willebrand activities, as demonstrated by its role in blood clotting and platelet aggregation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Don't Sweat the Swelling: Exercise Volume's Transient Effects in Trained Men.

    International journal of sports medicine·2026
    Same author

    Match Demands and Perceived Exertion of Cerebral Palsy Soccer National Team Players.

    Sports health·2025
    Same author

    Development of microsatellite markers for an outbreaking species of oak gall wasp, <i>Zapatella davisae</i> (Hymenoptera: Cynipidae), in the northeastern United States.

    Bulletin of entomological research·2018
    Same author

    Asymmetric hybridization between non-native winter moth, Operophtera brumata (Lepidoptera: Geometridae), and native Bruce spanworm, Operophtera bruceata, in the Northeastern United States, assessed with novel microsatellites and SNPs.

    Bulletin of entomological research·2016
    Same author

    Armored scale insect endosymbiont diversity at the species level: genealogical patterns of Uzinura diasipipdicola in the Chionaspis pinifoliae-Chionaspis heterophyllae species complex (Hemiptera: Coccoidea: Diaspididae).

    Bulletin of entomological research·2014
    Same author

    Eltrombopag as steroid sparing therapy for immune thrombocytopenic purpura in systemic lupus erythematosus.

    Lupus·2014

    Area of Science:

    • Biochemistry
    • Hematology

    Background:

    • Factor VIII is crucial for blood coagulation.
    • Hemophilia A is a genetic disorder characterized by deficient Factor VIII.
    • Von Willebrand factor (vWF) plays a role in platelet adhesion and Factor VIII stabilization.

    Purpose of the Study:

    • To investigate the molecular structure of human Factor VIII.
    • To determine if the procoagulant and von Willebrand activities reside on the same molecule.
    • To elucidate the subunit composition and assembly of Factor VIII.

    Main Methods:

    • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing and non-reducing conditions.
    • Gel chromatography on agarose in varying salt and calcium concentrations.
    • Analysis of protein subunits, molecular weight, and biological activities (procoagulant and platelet aggregation).

    Main Results:

    • Factor VIII exists as a large glycoprotein with identical 195,000-molecular-weight subunits linked by disulfide bonds.
    • Thrombin activation/inactivation and trypsin cleavage alter Factor VIII activity but not subunit size.
    • Plasmin degrades Factor VIII subunits into smaller peptides.
    • Both procoagulant and von Willebrand activities are associated with the same molecule, separable by calcium-dependent chromatography.

    Conclusions:

    • Human Factor VIII is a single large glycoprotein responsible for both antihemophilic and von Willebrand activities.
    • The molecule is composed of identical 195,000-molecular-weight subunits.
    • Calcium ions play a role in the dissociation of Factor VIII activities.

    Related Experiment Videos