Leptin receptor isoform expression in rat osteoblasts and their functional analysis
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Published on: August 4, 2023
Yun-Jung Lee1, Jung-Hyun Park, Sung-Kyu Ju
1Laboratory for the Study of Molecular Biointerface, Department of Oral Anatomy, College of Dentistry and Intellectual Biointerface Engineering Center (IBEC), BK21 HLS, Seoul National University, Yeonkun-Dong, Chongro-Ku, South Korea.
Abstract:
The genetic defect in producing the adipose hormone leptin results among others in a drastic increase of bone mass. The current understanding is that under normal circumstances, osteoblast activity is indirectly suppressed by a hypothalamic relay induced by leptin-signalling in the brain. To investigate whether leptin might also regulate osteoblast activity in a direct manner, expression of leptin receptors in rat osteoblasts was determined and their functionality was analyzed upon recombinant leptin treatment. Reverse transcription-PCR confirmed the expression of four among the six currently described receptor isoforms, which were also able to transduce cell signalling as shown by STAT3 phosphorylation after activation.
