Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thymosin beta4 promotes human conjunctival epithelial cell migration.

Gabriel Sosne1, Saif Hafeez, Albert L Greenberry

  • 1Kresge Eye Institute, Detroit, MI, USA.

Current Eye Research
|September 27, 2002
PubMed
Summary

Thymosin beta4 (Tbeta4) significantly enhances conjunctival cell migration and laminin-5 production. This peptide promotes healing by increasing extracellular matrix deposition and cell motility.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reparative Outcomes in Corneal Infection: Linking Adjunctive Tβ4 Treatment to Nerve Regeneration and Visual Function.

Investigative ophthalmology & visual science·2026
Same author

Activation of pro-resolving pathways mediate the therapeutic effects of thymosin beta-4 during <i>Pseudomonas aeruginosa</i>-induced keratitis.

Frontiers in immunology·2024
Same author

Decline in a Colonoscopist's Adenoma Detection Rate After First Cataract Surgery With Restoration After Second Cataract Surgery.

Gastro hep advances·2024
Same author

A Novel Combination Therapy Tβ4/VIP Protects against Hyperglycemia-Induced Changes in Human Corneal Epithelial Cells.

Biosensors·2023
Same author

Thymosin beta 4: A potential novel adjunct treatment for bacterial keratitis.

International immunopharmacology·2023
Same author

0.1% RGN-259 (Thymosin ß4) Ophthalmic Solution Promotes Healing and Improves Comfort in Neurotrophic Keratopathy Patients in a Randomized, Placebo-Controlled, Double-Masked Phase III Clinical Trial.

International journal of molecular sciences·2023

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Biochemistry

Background:

  • Conjunctival wound healing is crucial for ocular surface integrity.
  • Cell migration and extracellular matrix (ECM) production are key processes in healing.
  • Thymosin beta4 (Tbeta4) is a known regulator of cell migration and actin dynamics.

Purpose of the Study:

  • To investigate the effect of Tbeta4 on human conjunctival cell migration.
  • To analyze Tbeta4's influence on laminin-5 production in conjunctival cells.
  • To understand the role of Tbeta4 in the context of ocular surface repair.

Main Methods:

  • Boyden chamber assays were used to quantify Tbeta4-induced cell migration.
  • Immunofluorescence microscopy assessed focal adhesions (vinculin) and laminin-5.

Related Experiment Videos

  • Western blot analysis determined the expression of laminin-5 alpha3 and gamma2 chains.

    • Main Results:

    • Tbeta4 demonstrated a dose-dependent stimulation of conjunctival cell migration.
    • Tbeta4 treatment altered focal adhesion morphology, leading to smaller, rounded structures.
    • Increased expression of laminin-5 alpha3 and gamma2 chain proteins was observed in Tbeta4-treated cells.

    Conclusions:

    • Tbeta4 promotes in vitro migration of human conjunctival epithelial cells.
    • Tbeta4 influences focal adhesion dynamics and enhances laminin-5 deposition.
    • The observed increase in cell migration may be linked to elevated laminin-5 production by Tbeta4.