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Oubain-sensitive adenosine triphosphatase from human kidneys.

B R Nechay, J A Nelson, R R Contreras

The Journal of Pharmacology and Experimental Therapeutics

|February 1, 1975

Summary

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Ouabain-sensitive ATPase activity varies in human kidney sections, being highest in the medulla. Cortical enzyme activity decreases over time during kidney preservation for transplantation.

Area of Science:

Nephrology
Biochemistry
Transplantation Science

Background:

Kidney preservation is critical for transplantation success.
Adenosine triphosphatase (ATPase) activity is vital for kidney function.
Understanding ATPase in preserved kidneys informs viability assessment.

Purpose of the Study:

To investigate ouabain-sensitive ATPase activity in different regions of human cadaver kidneys.
To assess the impact of preservation time on cortical ATPase activity.
To characterize the kinetic properties and inhibition of kidney ATPase.

Main Methods:

Tissue homogenates and subcellular fractions from preserved human cadaver kidneys were analyzed.
Ouabain-sensitive ATPase activity was measured in cortical, medullary, and papillary tissues.

Related Experiment Videos

Enzyme kinetics and inhibition by ouabain and other agents were studied.

Main Results:

Ouabain-sensitive ATPase activity was highest in the medulla, intermediate in the cortex, and lowest in the papilla.
Cortical ATPase activity declined during maintenance perfusion.
Kinetic properties and ouabain sensitivity were similar between cortical and medullary enzymes, with K+ antagonism observed.

Conclusions:

Regional differences in ATPase activity exist in human kidneys.
Decreased cortical ATPase activity during preservation may indicate reduced kidney viability.
ATPase is a potential biomarker for assessing kidney quality in transplantation.