The use of construct variation and diffraction data analysis in the crystallization of the TRAF domain of human tumor
Hong Ye1, Maurizio Cirilli, Hao Wu
1Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021, USA.
Acta Crystallographica. Section D, Biological Crystallography
|September 28, 2002
Abstract:
Obtaining soluble proteins and diffraction-quality crystals often represents the bottleneck in macromolecular crystallography. Here, it is shown that construct variation can be an efficient strategy in expressing soluble proteins from bacteria and that clues from poor crystals may be used to improve crystal packing and to optimize crystal quality.


