Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Development and characterization of temperature-controlled microreactors for protein crystallization.

Michael Berg1, Matthias Urban, Ulrich Dillner

  • 1Institute for Physical High Technology, Winzerlaer Strasse 10, 07745 Jena, Germany. michael.berg@ipht-jena.de

Acta Crystallographica. Section D, Biological Crystallography
|September 28, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

National-scale prediction of arsenic, fluoride and its co-occurrence in groundwater of Mexico: Implications for drinking-water.

Journal of hazardous materials·2026
Same author

Click Capture SELEX for the Identification of Click-Modified Aptamers Targeting Small Molecules in Solution.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Enhanced HBcAg immunoassay enables detection of clinically relevant low-level HBV viremia for treatment eligibility.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology·2026
Same author

Groundwater arsenic in Taiwan: From Mid-20th-Century crisis to predictive models for risk mitigation strategies.

Environment international·2026
Same author

Selection and Characterization of SARS-CoV-2 Spike Binding Clickmers.

Chembiochem : a European journal of chemical biology·2026
Same author

Lighting the spark of aptamer data science.

Science (New York, N.Y.)·2026

We developed a novel microreactor for precise temperature control during biological macromolecule crystallization. This device enables in situ observation, facilitating the study of physico-chemical properties for high-quality crystal growth.

Area of Science:

  • Biophysical Chemistry
  • Crystallography
  • Biotechnology

Background:

  • Understanding physico-chemical properties is crucial for efficient biological macromolecule crystallization.
  • High-quality crystals are essential for structural determination of biomolecules.

Purpose of the Study:

  • To conceptualize and realize a novel temperature-controlled microreactor.
  • To study the physico-chemical parameters influencing crystallization.
  • To enable in situ observation of crystallization processes.

Main Methods:

  • Design and fabrication of a temperature-controlled microreactor.
  • Characterization of temperature distribution within the reaction chamber.
  • Assessment of device's long-term stability and accuracy.

Related Experiment Videos

  • Evaluation of surface regeneration after protein contamination (recombinant green fluorescent protein - rGFP).

    • Main Results:

    • A functional microreactor for temperature-controlled batch crystallization was successfully developed.
    • The device demonstrated accurate temperature distribution and stability.
    • Effective surface regeneration protocols were established for the microreactor.

    Conclusions:

    • The developed microreactor is suitable for in situ observation of temperature-controlled batch crystallization.
    • This technology aids in studying crystallization parameters for improved crystal quality.
    • The microreactor offers a valuable tool for biophysical and crystallographic research.