Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Downstream processing in the biotechnology industry.

Manohar Kalyanpur1

  • 1Bioseparations and Pharmaceutical Validation, Plaisir, France.

Molecular Biotechnology
|October 2, 2002
PubMed
Summary

This review covers techniques for recovering therapeutic proteins produced by biotechnology, from initial clarification to final purification. It also discusses process validation and contaminant removal according to regulatory guidelines.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

Applications of Recombinant DNA Technology in Medicine: A Comprehensive Review.

Molecular biotechnology·2026
Same journal

Optimizing miRNA Loading into Platelet Exosomes via Electroporation: A Comparative Analysis of Buffer Systems and Voltage Parameters for Enhanced Targeted Drug Delivery.

Molecular biotechnology·2026
Same journal

Regulation of Goat Milk Protein Synthesis: Genetic Architecture, Signalling Pathways, and Omics Insights.

Molecular biotechnology·2026
Same journal

Cloning and Functional Characterization of AhyAP-T65Lig, an ATP-Dependent DNA Ligase from Trabzonvirus AP-T65.

Molecular biotechnology·2026
Same journal

Overexpression of the ATP-Citrate Lyase Gene Enhances Ganoderic Acid Biosynthesis in Ganoderma lingzhi.

Molecular biotechnology·2026
Same journal

CRISPR/Cas9 Mediated Genome Editing for Enhancing Abiotic Stress Tolerance in Rice: An Omics Guided Perspective.

Molecular biotechnology·2026

Area of Science:

  • Biotechnology
  • Bioprocessing
  • Protein Therapeutics

Background:

  • The biotechnology industry utilizes recombinant microorganisms, cell lines, and animals to produce valuable therapeutic proteins.
  • Efficient recovery of these proteins from complex biological mixtures is crucial for their therapeutic application.

Purpose of the Study:

  • To review current techniques for the recovery of high-value therapeutic proteins in the biotechnology industry.
  • To discuss process validation and contaminant removal in the context of regulatory requirements.

Main Methods:

  • Review of established protein recovery methods, including centrifugation and membrane filtration for initial clarification.
  • Analysis of advanced purification techniques such as membrane-based and chromatographic methods.
  • Examination of process validation strategies for ensuring product safety and efficacy.

Main Results:

  • A comprehensive overview of the steps involved in therapeutic protein recovery, from upstream processing to downstream purification.
  • Emphasis on the importance of selecting appropriate methods for efficient and scalable protein isolation.
  • Discussion of critical aspects of process validation, including the removal of bacterial and viral contaminants.

Conclusions:

  • Effective protein recovery relies on a combination of clarification, purification, and validation steps.
  • Adherence to regulatory guidelines is essential for ensuring the safety and quality of therapeutic protein products.
  • Continuous improvement in recovery techniques is vital for the advancement of the biotechnology industry.

Related Experiment Videos