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Related Experiment Videos

A sample injection device for flow cytometers.

Harald B Steen1

  • 1Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway. h.b.steen@labmed.uio.no

Cytometry
|October 3, 2002
PubMed
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This study introduces a novel syringe-based system for flow cytometer sample injection, improving flushing efficiency and maintaining precise cell concentration measurements. The new device offers enhanced speed and accuracy for sample handling in flow cytometry.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Flow cytometry sample injection systems traditionally use pressure differential or volumetric methods.
  • Pressure differential systems offer rapid flushing but lack flow rate accuracy.
  • Volumetric systems provide accurate cell concentration but have inefficient flushing.

Purpose of the Study:

  • To develop an improved sample injection system for flow cytometers.
  • To combine the benefits of rapid flushing and accurate volumetric sample delivery.

Main Methods:

  • A removable syringe system was designed, connecting directly to sample tubing with a tight seal.
  • This design eliminates the need for a valve, streamlining the injection process.

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Main Results:

  • The developed device ensures highly constant sample flow rates, directly proportional to syringe velocity.
  • Flow rates ranging from 0.2-50 microl/min were achieved with a rapid 2-second settling time.

Conclusions:

  • The new system matches the precision of conventional flow cytometer injection methods.
  • Significant improvements in flushing speed and efficiency were demonstrated.