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Method for determining nitrogenous heterocycle compounds in wine.

Harald Keim1, Gilles de Revel, Stéphanie Marchand

  • 1Faculté d'OEnologie, Unité Associée INRA/Université Victor Segalen Bordeaux 2, 351 Cours de la Libération, F-33405 Talence Cedex, France.

Journal of Agricultural and Food Chemistry
|October 3, 2002
PubMed
Summary

This study identifies and quantifies N-heterocycle compounds in wine using gas chromatography. The developed method provides reliable results for analyzing wine

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Area of Science:

  • Food Chemistry
  • Analytical Chemistry
  • Organic Chemistry

Background:

  • N-heterocyclic compounds contribute to wine aroma and flavor.
  • Accurate identification and quantification of these compounds are crucial for wine quality assessment.

Purpose of the Study:

  • To develop and validate a method for the qualitative and quantitative analysis of N-heterocycles in wine.
  • To identify specific N-heterocyclic compounds in various wine samples.

Main Methods:

  • Liquid-liquid extraction for initial sample preparation.
  • Silica gel chromatography for purification of N-heterocycles.
  • Gas chromatography coupled with a nitrogen-phosphorus detector (GC-NPD) for analysis.
  • Gas chromatography-mass spectrometry (GC-MS) for compound identification.

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Main Results:

  • Identified and quantified 2,4,5-trimethyloxazole, 2,4-dimethylthiazole, and 4-methylthiazole using GC-NPD.
  • Identified 2-acetylthiazole and 2-acetyl-2-thiazoline using GC-MS.
  • The analytical procedure demonstrated reliability and reproducibility across different wine samples.

Conclusions:

  • The combined techniques of liquid-liquid extraction, silica gel purification, GC-NPD, and GC-MS provide an effective method for N-heterocycle analysis in wine.
  • This method enables accurate identification and quantification of key aroma-contributing compounds in wine.
  • The validated procedure is suitable for routine analysis in oenology and quality control.