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Published on: May 3, 2017
Effect of glutamate on arachidonic acid production from Mortierella alpina
1School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China. lanwz73@sohu.com
Aims:
To evaluate the effect of glutamate on arachidonic acid production from Mortierella alpina.
Methods And Results:
Cell growth, arachidonic acid production, proportions of poly-unsaturated fatty acids (PuFAs) in fatty acids and glucose-6-phosphate dehydrogenase (G6PDH) activity were analysed when glutamate concentration was 0.8 g l(-1). Biomass and arachidonic acid production were higher in the culture containing glutamate than those in the control culture, and both reached their maximum of 25 g l(-1) and 1.4 g l(-1) after 7 d, respectively. The proportions of some PuFAs, oleic acid, gamma-linolenic acid and dihomo-gamma-linolenic acid were decreased while linoleic acid and arachidonic acid were enhanced by glutamate addition. Glutamate addition enhanced G6PDH activity compared with the control during the whole culture process.
Conclusions:
Addition of 0.8 g l(-1) glutamate was beneficial to enhance arachidonic acid production from Mortierella alpina, which was a result of activating the pentose phosphate pathway (PPP).
Significance And Impact Of The Study:
This study shows that the addition of glutamate and regulation of PPP had a positive influence on arachidonic acid synthesis in Mortierella species.
